Lenti-mEGFP-hsG3BP1 delet RBD

LOCUS       Lenti-mEGFP-hsG3BP1 delet RBD              9907 bp DNA     circular SYN 11-DEC-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9907)
  AUTHORS   Yoo AS, Sun AX, Li L, Shcheglovitov A, Portmann T, Li Y, Lee-Messer 
            C, Dolmetsch RE, Tsien RW, Crabtree GR
  TITLE     MicroRNA-mediated conversion of human fibroblasts to neurons.
  JOURNAL   Nature. 2011 Jul 13. doi: 10.1038/nature10323.
  PUBMED    21753754
REFERENCE   2  (bases 1 to 9907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 2, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2011 Jul 13. doi: 10.1038/nature10323."
FEATURES             Location/Qualifiers
     source          1..9907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     misc_feature    2202..2209
                     /label=enzyme cut site
     enhancer        2210..2513
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2514..2717
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2756..2765
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2762..3478
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3497..4474
                     /codon_start=1
                     /label=hsG3BP1——delete RBD Domain
                     /translation="KLMVMEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYVHG
                     GLDSNGKPADAVYGQKEIHRKVMSQNFTNCHTKIRHVDAHATLNDGVVVQVMGLLSNNN
                     QALRRFMQTFVLAPEGSVANKFYVHNDIFRYQDEVFGGFVTEPQEESEEEVEEPEERQQ
                     TPEVVPDDSGTFYDQAVVSNDMEEHLEEPVAEPEPDPEPEPEQEPVSEIQEEKPEPVLE
                     ETAPEDAQKSSSPAPADIAQTVQEDLRTFSWASVTSKNLPPSGAVPVTGIPPHVVKVPA
                     SQPRPESKPESQIPPQRPQRDQRVREQRINIPPQRGPRPIREAG"
     misc_feature    complement(4436..4474)
                     /label=Primer R
     misc_feature    4454..4492
                     /label=Primer F
     misc_feature    4475..4480
                     /label=enzyme cut site
     promoter        4485..4984
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4520..4543)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4917..4936
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5011..5805
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5065..5084)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5675..5694
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    5829..6417
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5882..5902)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6300..6311)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6625..7258
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7387..7403)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7387..7403)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7400..7422)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7411..7427
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7435..7465)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7480..7501
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7618..7635)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7789..8374)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7869..7888)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8545..9405)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9168..9187
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9406..9510)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9558..9692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9595..9614)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9649..9668
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggc gttacataac
     2221 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     2281 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     2341 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     2401 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     2461 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     2521 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     2581 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     2641 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     2701 tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac cggtcgccac
     2761 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     2821 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     2881 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     2941 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     3001 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     3061 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     3121 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     3181 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     3241 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     3301 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     3361 ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt
     3421 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     3481 ctcagatctc gagctcaagc ttatggtgat ggagaagcct agtcccctgc tggtcgggcg
     3541 ggaatttgtg agacagtatt acacactgct gaaccaggcc ccagacatgc tgcatagatt
     3601 ttatggaaag aactcttctt atgtccatgg gggattggat tcaaatggaa agccagcaga
     3661 tgcagtctac ggacagaaag aaatccacag gaaagtgatg tcacaaaact tcaccaactg
     3721 ccacaccaag attcgccatg ttgatgctca tgccacgcta aatgatggtg tggtagtcca
     3781 ggtgatgggg cttctctcta acaacaacca ggctttgagg agattcatgc aaacgtttgt
     3841 ccttgctcct gaggggtctg ttgcaaataa attctatgtt cacaatgata tcttcagata
     3901 ccaagatgag gtctttggtg ggtttgtcac tgagcctcag gaggagtctg aagaagaagt
     3961 agaggaacct gaagaaagac agcaaacacc tgaggtggta cctgatgatt ctggaacttt
     4021 ctatgatcag gcagttgtca gtaatgacat ggaagaacat ttagaggagc ctgttgctga
     4081 accagagcct gatcctgaac cagaaccaga acaagaacct gtatctgaaa tccaagagga
     4141 aaagcctgag ccagtattag aagaaactgc ccctgaggat gctcagaaga gttcttctcc
     4201 agcacctgca gacatagctc agacagtaca ggaagacttg aggacatttt cttgggcatc
     4261 tgtgaccagt aagaatcttc cacccagtgg agctgttcca gttactggga taccacctca
     4321 tgttgttaaa gtaccagctt cacagccccg tccagagtct aagcctgaat ctcagattcc
     4381 accacaaaga cctcagcggg atcaaagagt gcgagaacaa cgaataaata ttcctcccca
     4441 aaggggaccc agaccaatcc gtgaggctgg ttgagaattc taccgggtag gggaggcgct
     4501 tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac
     4561 aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt
     4621 ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc
     4681 cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt
     4741 gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata
     4801 gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg
     4861 gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt
     4921 ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct
     4981 ttcgacctgc agcccaagct tcctaggacc atgattgaac aagatggatt gcacgcaggt
     5041 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     5101 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     5161 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg
     5221 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     5281 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     5341 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     5401 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     5461 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     5521 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     5581 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     5641 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     5701 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     5761 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgacccgg gcggggcgcg
     5821 tctggaacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     5881 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     5941 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     6001 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     6061 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     6121 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     6181 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     6241 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     6301 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     6361 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     6421 gaattaattc tgcagtcgag acctagaaaa acatggagca atcacaagta gcaatacagc
     6481 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggtttttc
     6541 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     6601 actttttaaa agaaaagagg ggactggaag ggctaattca ctcccaacga agacaagata
     6661 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     6721 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     6781 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     6841 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     6901 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     6961 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     7021 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     7081 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     7141 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     7201 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     7261 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     7321 agtgagaggc cttgacattg ctagcgtttt accgtcgacc tctagctaga gcttggcgta
     7381 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     7441 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     7501 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     7561 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     7621 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     7681 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     7741 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     7801 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     7861 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     7921 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     7981 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     8041 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     8101 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     8161 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     8221 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     8281 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggttttt ttgtttgcaa
     8341 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8401 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     8461 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     8521 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8581 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8641 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     8701 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8761 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8821 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     8881 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8941 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9001 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9061 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9121 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     9181 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9241 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9301 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9361 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     9421 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     9481 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     9541 cgacggatcg ggagatcaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     9601 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     9661 aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc
     9721 atcccaaact tcccacccca taccctatta ccactgccaa ttacctagtg gtttcattta
     9781 ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata
     9841 tgtactacaa acttagtagt ttttaaagaa attgtatttg ttaaatatgt actacaaact
     9901 tagtagt
//